The Histone H3 primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting target proteins in nuclear extracts.
The expression of Histone H3, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, Histone H3 primary antibodies can be used for the detection of Histone H3 when performing multiplex Western blot detection.
Detect Histone H3 Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
Histone H3 antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium="">
Do not aliquot the antibody.
| Properties | Histone H3 Mouse Monoclonal Antibody (P/N 926-42218) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 17 kDa |
| Isotype | Mouse IgG3 |
| Specificity/Sensitivity | Detects endogenous levels of total Histone H3 protein |
| Immunogen | A peptide that is specific to the carboxy terminus of human histone H3 protein |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F), Chromatin Immunoprecipitation (CHIP) |
RRID
- P/N 926-42218: RRID AB_2814903
Detection of Histone H3 Mouse Monoclonal Antibody in HeLa, NIH/3T3, and COS-7 Lysates
美国LI-COR公司成立于1971年，其产品广泛应用于全球的100多个国家，已成为从事植物生理及生态学研究的科学家的首选仪器。LI-COR公司的生态仪器应用于农学、生物技术、植物学、生态学、林业、园艺、湖沼生物学、气象学、海洋学、光学研究、植物生理学和日光研究等不同学科。主要产品有光合作用测定系统（LI-6400）、CO2/H2O气体分析系统（LI-7000/LI-7500）、CO2气体分析系统（LI-820）、稳态气孔计（LI-1600）、叶面积仪（LI-3100/LI-3000A）、植物树冠分析仪（LAI-2000）、露点发生器（LI-610）、数据采集器（LI-1400）、光强表（LI-250）、气象站（LI-1401/LI-1405）和各种光量子传感器等。
LI-COR公司的产品正是国际生态学研究的最佳仪器，无论是研究成果的发表，还是应用，使用LI-COR产品与否，已体现到其结果的可靠性和真实性。有人对国际著名生态学杂志作过粗略统计，凡涉及到使用LI-COR产品或其同类厂家仪器的文章中，以使用LI-COR产品的文章最多，约占70%以上。
本网站将在规定时间内给予删除等相关处理。
