IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
Recommended Dilutions
| Application | Suggested Range | Tween 20* | SDS* |
|---|---|---|---|
| Odyssey® Western blot detection | 1:1,000 - 1:5,000 | 0.1 - 0.2% (v/v) | 0.02 - 0.1% (v/v) |
| Other | User optimized | User optimized | User optimized |
Optimum dilutions will vary and should be determined empirically.
* Added to reduce non-specific background staining.
See the complete line of IRDye Streptavidins.
美国LI-COR公司成立于1971年，其产品广泛应用于全球的100多个国家，已成为从事植物生理及生态学研究的科学家的首选仪器。LI-COR公司的生态仪器应用于农学、生物技术、植物学、生态学、林业、园艺、湖沼生物学、气象学、海洋学、光学研究、植物生理学和日光研究等不同学科。主要产品有光合作用测定系统（LI-6400）、CO2/H2O气体分析系统（LI-7000/LI-7500）、CO2气体分析系统（LI-820）、稳态气孔计（LI-1600）、叶面积仪（LI-3100/LI-3000A）、植物树冠分析仪（LAI-2000）、露点发生器（LI-610）、数据采集器（LI-1400）、光强表（LI-250）、气象站（LI-1401/LI-1405）和各种光量子传感器等。
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